Effect of 1α,25(OH)2D3-Treated M1 and M2 Macrophages on Cell Proliferation and Migration Ability in Ovarian Cancer

• Published in: Nutrition and cancer Vol. 74; no. 7; pp. 2632 - 2643
• Main Authors: Guo, Yi, Jiang, Fei, Yang, Wenqing, Shi, Weiqiang, Wan, Jianmei, Li, Jie, Pan, Jinjing, Wang, Ping, Qiu, Junlan, Zhang, Zengli, Li, Bingyan
• Format: Journal Article
• Language: English
• Published: Mahwah Taylor & Francis 2022; Taylor & Francis Ltd
• Subjects: Acetic acid; Anticancer properties; Bone tumors; Cancer; Cell differentiation; Cell growth; Cell proliferation; Gelatinase B; Immunomodulation; Immunomodulators; Leukocyte migration; Macrophages; Metastases; Mineralization; Monocytes; Ovarian cancer; Phorbol 12-myristate 13-acetate; Prognosis; Transforming growth factor-b1; Tumorigenesis; Vitamin D3
• ISSN: 0163-5581; 1532-7914
• DOI: 10.1080/01635581.2021.2014903

The biological active form of vitamin D3, 1α,25-dehydroxyvitamin D3 [1α,25(OH) 2 D 3 ], exerts pleiotropic effects including bone mineralization, anti-tumor, as well as immunomodulator. This study aimed to explore the potential impact of 1α,25(OH) 2 D 3 on tumor-associated macrophages (TAMs) infiltration in ovarian cancer. Firstly, human monocytic THP-1 cells were differentiated into macrophages (M0) in the presence of phorbol 12-myristate 13-acetate (PMA). In Vivo, 1α,25(OH) 2 D 3 not only reversed the polarization of M2 macrophages, but also decreased the proliferation and migration abilities of ovarian cancer cells induced by M2 macrophages supernatant. Furthermore, 1α,25(OH) 2 D 3 dramatically decreased the secretion of TGF-β1 and MMP-9 in M2 macrophages. However, no significant effect was observed in 1α,25(OH) 2 D 3 treated M1 macrophages. In Vivo, vitamin D3 had an inhibitive effect of 1α,25(OH) 2 D 3 -treated M2 macrophages on tumorigenesis. In addition, we conducted the association of TAMs with the poor prognosis of patients with ovarian cancer by meta-analysis, which suggested the higher proportion of M2 macrophages was related to the poorer prognosis in ovarian cancer. Collectively, these results identified distinct roles of 1α,25(OH) 2 D 3 treated M1 and M2 macrophages on cell proliferation and migration abilities in ovarian cancer.