Root antioxidant responses of two Pisum sativum cultivars to direct and induced Fe deficiency

• Published in: Plant biology (Stuttgart, Germany) Vol. 16; no. 3; pp. 607 - 614
• Main Authors: Jelali, N., Donnini, S., Dell'Orto, M., Abdelly, C., Gharsalli, M., Zocchi, G.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.05.2014
• Subjects: Antioxidants - metabolism; Ascorbic Acid - metabolism; Catalase - metabolism; Glutathione - metabolism; Hydrazones - metabolism; Hydrogen Peroxide - metabolism; Iron - pharmacology; Iron Deficiencies; Iron deficiency; Isoenzymes - metabolism; lipid peroxidation; Lipid Peroxidation - drug effects; Malondialdehyde - metabolism; pea; Peroxidase - metabolism; peroxidase isoforms; Pisum sativum - enzymology; Pisum sativum - growth & development; Pisum sativum - metabolism; Pisum sativum - physiology; Plant Roots - enzymology; Plant Roots - growth & development; Plant Roots - metabolism; Plant Roots - physiology; root antioxidant enzymes; Substrate Specificity - drug effects; Superoxide Dismutase - metabolism; Iron deficiency; peroxidase isoforms; lipid peroxidation; pea; root antioxidant enzymes
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.12093

The contribution of antioxidant defence systems in different tolerance to direct and bicarbonate-induced Fe deficiency was evaluated in two pea cultivars (Kelvedon, tolerant and Lincoln, susceptible). Fe deficiency enhanced lipid peroxidation and H2 O2 concentration in roots of both cultivars, particularly in the sensitive one grown under bicarbonate supply. The results obtained on antioxidant activities (SOD, CAT, POD) suggest that H2 O2 accumulation could be due to an overproduction of this ROS and, at the same time, to a poor capacity to detoxify it. Moreover, under bicarbonate supply the activity of POD isoforms was reduced only in the sensitive cultivar, while in the tolerant one a new isoform was detected, suggesting that POD activity might be an important contributor to pea tolerance to Fe deficiency. The presence of bicarbonate also resulted in stimulation of GR, MDHAR and DHAR activities, part of the ASC-GSH pathway, which was higher in the tolerant cultivar than in the sensitive one. Overall, while in the absence of Fe only slight differences were reported between the two cultivars, the adaptation of Kelvedon to the presence of bicarbonate seems to be related to its greater ability to enhance the antioxidant response at the root level.