The polycotyledon (pct1-2) mutant of tomato shows enhanced accumulation of PIN1 auxin transport facilitator protein

• Published in: Plant biology (Stuttgart, Germany) Vol. 12; no. 1; pp. 224 - 228
• Main Authors: Kharshiing, E. V., Kumar, G. P., Ditengou, F. A., Li, X., Palme, K., Sharma, R.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.2010
• Subjects: Enhanced PIN1 accumulation; Gene Expression Regulation, Plant; Hypocotyl - metabolism; Indoleacetic Acids - metabolism; Lycopersicon esculentum - genetics; Lycopersicon esculentum - metabolism; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Mutation; PIN family proteins; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - metabolism; polar auxin transport; polycotyledony; Protein Transport; Solanum lycopersicum
• ISSN: 1435-8603; 1438-8677; 1438-8677
• DOI: 10.1111/j.1438-8677.2009.00267.x

The bilateral symmetry of a dicotyledon embryo is tightly associated with the directional flow of auxin. Disruption of polar auxin flow results in various developmental abnormalities. The pct1-2 mutant of tomato, showing polycotyledony, also has enhanced polar auxin transport in hypocotyls. Immunocytochemical analysis revealed increased PIN1 protein in pct1-2 roots and hypocotyls. The mutant also displayed an increase in PIN1 transcript levels in these organs. Our results indicate that over-accumulation of PIN1 protein is likely related to increased polar transport of auxin in the pct1-2 mutant.