Ezrin-dependent regulation of the actin cytoskeleton by β-dystroglycan

• Published in: Human molecular genetics Vol. 13; no. 15; pp. 1657 - 1668
• Main Authors: Spence, H.J., Chen, Y.-J., Batchelor, C.L., Higginson, J.R., Suila, H., Carpen, O., Winder, S.J.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.08.2004
• Subjects: Actins - metabolism; Binding Sites - genetics; Binding Sites - physiology; Biological and medical sciences; Cytoskeletal Proteins; Cytoskeleton - metabolism; Dystroglycans - genetics; Dystroglycans - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Reporter; Genetics of eukaryotes. Biological and molecular evolution; Humans; Molecular and cellular biology; Muscular Dystrophy, Duchenne - metabolism; Mutation; Phosphoproteins - genetics; Phosphoproteins - metabolism; Protein Binding; Cytoskeleton; Genetics; Regulation(control); Actin; Dystrophin
• ISSN: 0964-6906; 1460-2083
• DOI: 10.1093/hmg/ddh170

Dystroglycan is part of an adhesion receptor complex linking the extracellular matrix to the actin cytoskeleton. Previous studies have implicated dystroglycan in basement membrane formation and as a crucial link between dystrophin and laminin in muscle. We report here a further novel function for dystroglycan which appears to be in addition to its role as an adhesion molecule. β-dystroglycan has been localized to microvilli structures in a number of cell types where it associates with the cytoskeletal adaptor ezrin, through which it is able to modulate the actin cytoskeleton and induce peripheral filopodia and microvilli. Ezrin is able to interact with dystroglycan through a cluster of basic residues in the juxtamembrane region of dystroglycan, and mutation of these residues both prevents ezrin binding and the induction of actin-rich surface protrusions. These studies reveal novel functions and additional signalling roles for dystroglycan, raising the possibility of new avenues for therapeutic intervention in diseases such as Duchenne muscular dystrophy.