Glycosphingolipids of subcellular fractions from normal rat liver and Morris hepatoma 5123TC

Neutral glycosphingolipids and gangliosides were quantified in lipid extracts from plasma membranes, mitochondria, microsomes, and nuclei isolated from normal rat liver and Morris hepatoma 5123TC. Results showed a higher content of glycosphingolipids, especially gangliosides, in hepatomas and differ...

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Published inJNCI : Journal of the National Cancer Institute Vol. 62; no. 2; p. 367
Main Authors Dnistrian, A M, Skipski, V P, Barclay, M, Stock, C C
Format Journal Article
LanguageEnglish
Published United States 01.02.1979
Subjects
Animals
Cell Nucleus - metabolism
Gangliosides - metabolism
Glycosphingolipids - metabolism
Liver - metabolism
Liver Neoplasms, Experimental - metabolism
Membrane Lipids - metabolism
Microsomes, Liver - metabolism
Mitochondria, Liver - metabolism
Rats
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Summary:Neutral glycosphingolipids and gangliosides were quantified in lipid extracts from plasma membranes, mitochondria, microsomes, and nuclei isolated from normal rat liver and Morris hepatoma 5123TC. Results showed a higher content of glycosphingolipids, especially gangliosides, in hepatomas and differences in the distribution of glycosphingolipids among subcellular fractions. Differences in the glycosphingolipid composition of the hepatoma, namely, the absence of trisialogangliosides and an increase in the lower molecular weight gangliosides, reflected an altered metabolism of glycosphingolipids in this tumor. The results indicated that changes in membrane glycosphingolipids were not restricted to the cell surfaces of malignant cells, inasmuch as intracellular membrane fractions also exhibited altered glycosphingolipid profiles.
ISSN:0027-8874
DOI:10.1093/jnci/62.2.367