DETERMINATION OF HYDROXYPYRENE TRISULFONATE BY TWO WAVELENGTH EXCITATION FLUORESCENCE USING A ONE MICROLITER CAPILLARY

Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employi...

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Bibliographic Details
Published inInstrumentation science & technology Vol. 42; no. 6; pp. 627 - 634
Main Authors Flaxer, Eli, Fisherman, Boris, Tirosh, Reuven, Patolsky, Fernando
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis Group 02.11.2014
Taylor & Francis Ltd
Subjects
capillaries
fluorescence
spectroscopy
Spectrum analysis
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Summary:Fluorescence is a type of electromagnetic spectroscopy that employs emission from a sample. It involves using a beam of light that excites the electrons in specific compounds and causes them to emit light of a lower energy. As proof of principle, a computer controlled experiment is described employing a single microliter capillary tube setup, using a two wavelength excitation of a single wavelength emission of hydroxypyrene trisulfonate. The same setup can be manipulated for the other pH-sensitive fluorescence ratiometric systems. In addition, this setup can be scaled up for high-throughput measurements.
ISSN:1073-9149
1525-6030
DOI:10.1080/10739149.2014.942916