Up-Conversion Luminescent and Porous NaYF4:Yb3+, Er3+@SiO2 Nanocomposite Fibers for Anti-Cancer Drug Delivery and Cell Imaging

• Published in: Advanced functional materials Vol. 22; no. 13; pp. 2713 - 2722
• Main Authors: Hou, Zhiyao, Li, Chunxia, Ma, Ping'an, Cheng, Ziyong, Li, Xuejiao, Zhang, Xiao, Dai, Yunlu, Yang, Dongmei, Lian, Hongzhou, Lin, Jun
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 10.07.2012; WILEY‐VCH Verlag
• Subjects: cell imaging; drug delivery; electrospinning; porous SiO2 fibers; up-conversion
• ISSN: 1616-301X; 1616-3028
• DOI: 10.1002/adfm.201200082

Up‐conversion (UC) luminescent and porous NaYF4:Yb3+, Er3+@SiO2 nanocomposite fibers are prepared by electrospinning process. The biocompatibility test on L929 fibrolast cells reveals low cytotoxicity of the fibers. The obtained fibers can be used as anti‐cancer drug delivery host carriers for investigation of the drug storage/release properties. Doxorubicin hydrochloride (DOX), a typical anticancer drug, is introduced into NaYF4:Yb3+, Er3+@SiO2 nanocomposite fibers (denoted as DOX‐NaYF4:Yb3+, Er3+@SiO2). The release properties of the drug carrier system are examined and the in vitro cytotoxicity and cell uptake behavior of these NaYF4:Yb3+, Er3+@SiO2 for HeLa cells are evaluated. The release of DOX from NaYF4:Yb3+, Er3+@SiO2 exhibits sustained, pH‐sensitive release patterns and the DOX‐NaYF4:Yb3+, Er3+@SiO2 show similar cytotoxicity as the free DOX on HeLa cells. Confocal microscopy observations show that the composites can be effectively taken up by HeLa cells. Furthermore, the fibers show near‐infrared UC luminescence and are successfully applied in bioimaging of HeLa cells. The results indicate the promise of using NaYF4:Yb3+, Er3+@SiO2 nanocomposite fibers as multi‐functional drug carriers for drug delivery and cell imaging. NaYF4:Yb3+, Er3+@SiO2 nanocomposite fibers, which are used as a novel anticancer drug carrier, are prepared using an electrospinning process. The fibers show the attractive properties of regular morphology, porous structure, good biocompatibility, and up‐conversion emission. The drug loading and release properties, cytotoxicity, cellular uptake, and cell imaging of nanocomposite fibers are investigated.