Occurrence of Ditaurobilirubin, Bilirubin Conjugated with Two Moles of Taurine, in the Gallbladder Bile of Yellowtail, Seriola quinqueradiata

• Published in: Journal of biochemistry (Tokyo) Vol. 102; no. 4; pp. 793 - 796
• Main Authors: SAKAI, Tadashi, WATANABE, Katsuko, KAWATSU, Hiroshi
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.10.1987
• Subjects: Animals; Azo Compounds - analysis; Azo Compounds - isolation & purification; Bile - analysis; Bilirubin - analogs & derivatives; Bilirubin - analysis; Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Fishes - metabolism; Gallbladder - metabolism; Indicators and Reagents; Marine; Taurine - analogs & derivatives; Taurine - analysis; Seriola quinqueradiata
• ISSN: 0021-924X
• DOI: 10.1093/oxfordjournals.jbchem.a122117

An unknown bilirubin conjugate was detected by HPLC analysis in the bile of yellowtail,Seriola quinqueradiata. The retention time of this unknown conjugate was identical with that of authentic ditaurobilirubin, a bilirubin conjugated with 2 mol of taurine. The retention times of the ethyl anthranilate diazo derivatives of both bilirubin conjugates were the same. The azo derivatives of this unknown conjugate were purified by Amberlite XAD-2 treatment and preparative TLC and hydrolyzed with 6 N HC1 at 105°C for 12 h. The hydrolyzates obtained were analyzed with an amino acid analyzer. The ninhydrin-positive substance in the hydrolyzates was purified by column chromatography and analyzed by TLC and mass spectrometry. The results of these analyses indicated that this ninhydrin-positive substance was taurine. Therefore, this unknown bilirubin conjugate was identified as ditaurobilirubin.