Studies in red blood cell preservation. 5. Determining the limiting concentrations of NH4Cl and Na2HPO4 needed to maintain red blood cell ATP during storage

The purpose of the present study was to define the lowest concentrations of ammonium (NH4+) and phosphate (Pi) in an experimental additive solution (EAS) that would support suitable red blood cell (RBC) ATP levels and other in vitro characteristics for at least 84 days. It was determined that ATP ma...

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Published inVox sanguinis Vol. 62; no. 3; p. 136
Main Authors Dumaswala, U J, Oreskovic, R T, Petrosky, T L, Greenwalt, T J
Format Journal Article
LanguageEnglish
Published England 1992
Subjects
2,3-Diphosphoglycerate
Adenosine Triphosphate - blood
Ammonia - blood
Ammonium Chloride - blood
Ammonium Chloride - pharmacology
Blood Preservation
Diphosphoglyceric Acids - blood
Erythrocytes - drug effects
Hemolysis - drug effects
Humans
Phosphates - blood
Phosphates - pharmacology
Potassium - blood
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Summary:The purpose of the present study was to define the lowest concentrations of ammonium (NH4+) and phosphate (Pi) in an experimental additive solution (EAS) that would support suitable red blood cell (RBC) ATP levels and other in vitro characteristics for at least 84 days. It was determined that ATP maintenance was dependent upon both NH4+ and Pi concentrations. RBCs stored for 84 days in additive solutions containing 10 mM NH4+ and 0, 15, 25 and 40 mM Pi had ATP values averaging 1.87, 2.49, 2.70 and 2.65 mumol/g Hb, respectively. The shedding of exocytic hemoglobin-containing vesicles and percent hemolysis were significantly (p less than 0.001) elevated in the preservative containing 40 mM Pi. These data suggest that an EAS containing 10 mM NH4+ and 15 mM Pi would be optimal for storing RBCs up to 84 days. The extended storage would be particularly advantageous for autologous transfusion programs.
ISSN:0042-9007
DOI:10.1111/j.1423-0410.1992.tb01186.x