Preparation of Diphosphopyridine Nucleotide from Bakers' Yeast

A method for the preparatoin of diphosphopyridine nucleotide (DPN) from bakers' yeast is described. This method consists of partial purification of crude extract of yeast by charcoal chromatography according to Pontis and coworkers, ion-exchange chromatography on Dowex-l acetate, and precipitat...

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Published inAgricultural and biological chemistry Vol. 25; no. 1; pp. 39 - 44
Main Authors Harashima, Keiji, Takaichi, Osashi, Nakamura, Michinori
Format Journal Article
LanguageEnglish
Published Taylor & Francis 01.01.1961
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Summary:A method for the preparatoin of diphosphopyridine nucleotide (DPN) from bakers' yeast is described. This method consists of partial purification of crude extract of yeast by charcoal chromatography according to Pontis and coworkers, ion-exchange chromatography on Dowex-l acetate, and precipitation of DPN as the free acid with ethanol. 0.71~.1.1 g of DPN with a purity of 85~90% was obtained from 5 kg of fresh bakers' yeast by this method.
ISSN:0002-1369
DOI:10.1080/00021369.1961.10857771