1H-NMR-based metabolomic analysis of the effect of moderate wine consumption on subjects with cardiovascular risk factors

• Published in: Electrophoresis Vol. 33; no. 15; pp. 2345 - 2354
• Main Authors: Vázquez-Fresno, Rosa, Llorach, Rafael, Alcaro, Francesca, Rodríguez, Miguel Ángel, Vinaixa, Maria, Chiva-Blanch, Gemma, Estruch, Ramon, Correig, Xavier, Andrés-Lacueva, Cristina
• Format: Journal Article
• Language: English
• Published: Blackwell Publishing Ltd 01.08.2012
• Subjects: Biomarkers; Metabolomics; Nuclear Magnetic Resonance; Urinary profile; Wine intake
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.201100646

Moderate wine consumption is associated with health‐promoting activities. An H‐NMR‐based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty‐one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24‐h urine samples were collected and analyzed by 1H‐NMR. According to the results of a one‐way ANOVA, significant markers were grouped in four categories: alcohol‐related markers (ethanol); gin‐related markers; wine‐related markers; and gut microbiota markers (hippurate and 4‐hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched‐chain amino acid (BCAA) metabolite (3‐methyl‐oxovalerate). Additionally, a possible interaction between alcohol and gut‐related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake.