Nanostructured Microfluidic Sensor Surfaces for Selective Cellular Wicking and Enrichment

This work presents a technique for using nano and micro structured sensor surfaces for tailored yet label-free selective wicking, adhesion, and detection of dilute target cells. Differential cell adhesion was optimized for enrichment of fetal trophoblasts from clinical cervical cell (liquid Pap) sam...

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Bibliographic Details
Published in2023 IEEE BioSensors Conference (BioSensors) pp. 1 - 4
Main Authors Welch, E. Celeste, Flores, Riley Renee, Tripathi, Anubhav
Format Conference Proceeding
LanguageEnglish
Published IEEE 30.07.2023
Subjects
Adhesives
Biosensors
cell rolling
Gold
interface
label-free
microfluidics
nanoengineering
Performance evaluation
single-cell analysis
Surface impedance
Surface morphology
System-on-chip
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Summary:This work presents a technique for using nano and micro structured sensor surfaces for tailored yet label-free selective wicking, adhesion, and detection of dilute target cells. Differential cell adhesion was optimized for enrichment of fetal trophoblasts from clinical cervical cell (liquid Pap) samples in a patterned microfluidic chip. Hydrophobic polystyrene spherical microstructures of 10 ×m yielded optimal enrichment of dilute trophoblast cells of up to ~40,000% when compared with ~400% maximum performance on planar surfaces. Cellular enrichment was maintained or enhanced when surfaces were covered in a gold nano sputtered coating, creating composite hierarchical surfaces with adequate electrical conductivity. This indicates that the technique can be used in creating advanced cell enrichment and sensing platforms when combining the microfluidic enrichment technique with an impedance sensor technique. This device is potentially useful in rare cell enrichment and analysis.
DOI:10.1109/BioSensors58001.2023.10281101