Nanostructured Microfluidic Sensor Surfaces for Selective Cellular Wicking and Enrichment
This work presents a technique for using nano and micro structured sensor surfaces for tailored yet label-free selective wicking, adhesion, and detection of dilute target cells. Differential cell adhesion was optimized for enrichment of fetal trophoblasts from clinical cervical cell (liquid Pap) sam...
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Published in | 2023 IEEE BioSensors Conference (BioSensors) pp. 1 - 4 |
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Main Authors | , , |
Format | Conference Proceeding |
Language | English |
Published |
IEEE
30.07.2023
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Subjects | |
Online Access | Get full text |
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Summary: | This work presents a technique for using nano and micro structured sensor surfaces for tailored yet label-free selective wicking, adhesion, and detection of dilute target cells. Differential cell adhesion was optimized for enrichment of fetal trophoblasts from clinical cervical cell (liquid Pap) samples in a patterned microfluidic chip. Hydrophobic polystyrene spherical microstructures of 10 ×m yielded optimal enrichment of dilute trophoblast cells of up to ~40,000% when compared with ~400% maximum performance on planar surfaces. Cellular enrichment was maintained or enhanced when surfaces were covered in a gold nano sputtered coating, creating composite hierarchical surfaces with adequate electrical conductivity. This indicates that the technique can be used in creating advanced cell enrichment and sensing platforms when combining the microfluidic enrichment technique with an impedance sensor technique. This device is potentially useful in rare cell enrichment and analysis. |
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DOI: | 10.1109/BioSensors58001.2023.10281101 |