Microfabricated Isothermal Eg-Fet Sensor For Lamp Mediated Crispr/Cas12a Detection Of Hepatitis C Virus
A highly sensitive and rapid platform for the detection of hepatitis C virus (HCV) has been developed by combining loop-mediated isothermal amplification (LAMP) with the use of clustered regularly interspaced short palindromic repeats of associated protein 12 (CRISPR/cas12a). An isothermal sensor wh...
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Published in | 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems (MEMS) pp. 448 - 451 |
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Main Authors | , , , , |
Format | Conference Proceeding |
Language | English |
Published |
IEEE
15.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | A highly sensitive and rapid platform for the detection of hepatitis C virus (HCV) has been developed by combining loop-mediated isothermal amplification (LAMP) with the use of clustered regularly interspaced short palindromic repeats of associated protein 12 (CRISPR/cas12a). An isothermal sensor which serves as an indium tin oxide (ITO) based extended gate field-effect transistor (EG-FET) and is equipped with a polymethyl methacrylate (PMMA) microwell, is used to detect the potential change on the sensing surface, induced by the LAMP/CRISPR reaction. The immobilized signal reporter, which is single-strand deoxyribonucleic acid (ssDNA) on the ITO sensing surface, is cleaved by the activated CRISPR/cas12a. This trans-cleavage occurs due to the highly specific base-pairing of a nucleic acid target to a complementary guide ribonucleic acid (gRNA). The result is a significant potential change within four minutes. The diagnostic platform that is established in this study can be applied to the detection of other virus/bacteria through the use of different pathogen-specific LAMP/CRISPR assays. Therefore, this technique provides a rapid yet high performance platform for the detection of label-free pathogens. |
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ISSN: | 2160-1968 |
DOI: | 10.1109/MEMS49605.2023.10052240 |