New GABAA receptor alpha5 subunit gene polymorphism that may confound genotyping
We report the discovery of a new GABAA receptor alpha5 subunit gene polymorphism close to the polymorphism described by Glatt et al. (GT)5GCGTGC(GT)21. This new polymorphism is of great importance, because it means that non‐denaturing acrylamide gels used to separate the different alleles of the pol...
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Published in | American journal of medical genetics. Part B, Neuropsychiatric genetics Vol. 129B; no. 1; pp. 27 - 28 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
15.08.2004
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Subjects | |
Online Access | Get full text |
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Summary: | We report the discovery of a new GABAA receptor alpha5 subunit gene polymorphism close to the polymorphism described by Glatt et al. (GT)5GCGTGC(GT)21. This new polymorphism is of great importance, because it means that non‐denaturing acrylamide gels used to separate the different alleles of the polymorphism described by Glatt et al. cannot distinguish an allele with the sequence: (GT)4GCGTGC(GT)n from another allele with the sequence: (GT)4(GCGT)4GC(GT)n−6. These gel fragments are separated by size, which would be the same in these two cases. An alternative would be to use an analysis method that can detect base changes, for instance, single strand conformation polymorphism (SSCP) or denaturing gradient gel electrophoresis (DGGE). © 2004 Wiley‐Liss, Inc. |
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Bibliography: | istex:2E40ACF7250A2C7888194752D96EFF46DAB80C33 2001 National Alliance for Research on Schizophrenia and Affective Disorders (NARSAD) Young Investigator Award ArticleID:AJMG30048 Spanish Health Ministry - No. FIS 00/317 ark:/67375/WNG-703BZT0H-Z ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1552-4841 1552-485X |
DOI: | 10.1002/ajmg.b.30048 |