An injectable PC10ARGD/Cu2+/DOX hydrogel for combined chemodynamic and chemotherapy of tumors

Nowadays, chemotherapy is a common clinical treatment for cancer, but it still faces many limitations and challenges. Therefore, the combination of chemotherapy and other treatments often enhances the effectiveness of treatments. Herein, an injectable hydrogel of PC 10 ARGD/Cu 2+ /DOX based on Cu 2+...

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Bibliographic Details
Published inJournal of biomaterials science. Polymer ed. Vol. 35; no. 2; pp. 190 - 205
Main Authors Yang, Jie, Wang, Xiao-Jie, Li, Si-Mei, Jiang, Dong-Xue, Liu, Yu-Xin, Huang, Yan, Dong, Di-Di, Hu, Wei, Liu, Bo
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 01.02.2024
Taylor & Francis Ltd
Subjects
chemodynamic therapy
Chemotherapy
Hydrogels
Injectable hydrogel
Polypeptides
tumor
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Summary:Nowadays, chemotherapy is a common clinical treatment for cancer, but it still faces many limitations and challenges. Therefore, the combination of chemotherapy and other treatments often enhances the effectiveness of treatments. Herein, an injectable hydrogel of PC 10 ARGD/Cu 2+ /DOX based on Cu 2+ , hydrophilic doxorubicin (DOX), and genetically engineered polypeptide PC 10 ARGD was prepared. First, Cu 2+ was attached to the histidines in the PC 10 ARGD polypeptide by the coordination reaction to form PC 10 ARGD/Cu 2+ hydrogel, then the PC 10 ARGD/Cu 2+ /DOX hydrogel was prepared by encapsulating the DOX into the PC 10 ARGD/Cu 2+ hydrogel. The results of scanning electron microscopy showed that the PC 10 ARGD/Cu 2+ /DOX hydrogel displayed loose porous morphology. In vitro, reactive oxygen species production results showed that the PC 10 ARGD/Cu 2+ /DOX hydrogel could continuously produce ·OH in the presence of H 2 O 2 . In vitro MTT results showed that the PC 10 ARGD/Cu 2+ /DOX hydrogel had a good inhibitory effect on cell activity. Flow cytometry further confirmed the antitumor effect of the PC 10 ARGD/Cu 2+ /DOX hydrogel. In vivo experiment results showed that the tumor volume of mice treated with the PC 10 ARGD/Cu 2+ /DOX hydrogel was significantly inhibited compared with control groups, which was due to the combination of chemodynamic and chemotherapy. The results of body weight and blood analysis of mice showed that the PC 10 ARGD/Cu 2+ /DOX hydrogel possessed good biocompatibility.
ISSN:0920-5063
1568-5624
DOI:10.1080/09205063.2023.2273118