Extending Femtosecond Stimulated Raman Microscopy Toward Silent and Fingerprint Region of Biomolecules
In this work, the design and the implementation of a femtosecond Stimulated Raman Scattering microscope, equipped with three femtosecond laser sources: a Titanium-Sapphire (Ti:Sa), an optical parametric oscillator (OPO) and a second harmonic generator (SHG) is presented. Our microscope is designed i...
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Published in | 2020 22nd International Conference on Transparent Optical Networks (ICTON) pp. 1 - 4 |
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Main Authors | , , |
Format | Conference Proceeding |
Language | English |
Published |
IEEE
01.07.2020
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Subjects | |
Online Access | Get full text |
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Summary: | In this work, the design and the implementation of a femtosecond Stimulated Raman Scattering microscope, equipped with three femtosecond laser sources: a Titanium-Sapphire (Ti:Sa), an optical parametric oscillator (OPO) and a second harmonic generator (SHG) is presented. Our microscope is designed in such a manner which has the capability to cover all the regions of Raman spectra, taking advantage of two possible laser combinations. The first, Ti:Sa and OPO laser beams, which covers the C-H region or O-H region (F > 2800\ \mathrm{cm}^{-1} in stimulated Raman gain (SRG) modality, whereas the second, Ti:Sa and SHG laser beams, covers the C-H region and the fingerprint region ( < 1800\ \mathrm{cm}^{-1} ) and the silent region (1800 - 2800 cm −1 ) in stimulated Raman loss (SRL) modality. In order to demonstrate the successful realization of microscope, SRL and SRG images of polystyrene beads using the two-laser combination has been successfully reported. |
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ISSN: | 2161-2064 |
DOI: | 10.1109/ICTON51198.2020.9203024 |