3D localized 1H-13C heteronuclear single-quantum coherence correlation spectroscopy in vivo

• Published in: Magnetic resonance in medicine Vol. 43; no. 2; pp. 200 - 210
• Main Authors: Watanabe, H., Ishihara, Y., Okamoto, K., Oshio, K., Kanamatsu, T., Tsukada, Y.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.02.2000; Williams & Wilkins
• Subjects: 1H-13C HSQC correlation; amino acid metabolism; Animals; Biological and medical sciences; Brain - metabolism; Carbon Isotopes; Female; Glucose - administration & dosage; Glutamic Acid - metabolism; Glutamine - metabolism; Hydrogen; in vivo; Investigative techniques, diagnostic techniques (general aspects); Macaca fascicularis; Medical sciences; MRS; Nervous system; Nuclear Magnetic Resonance, Biomolecular - instrumentation; Nuclear Magnetic Resonance, Biomolecular - methods; Phantoms, Imaging; Radiodiagnosis. Nmr imagery. Nmr spectrometry; Reproducibility of Results; spatial localization; Time Factors; Hydrogen; Spatial correlation; Monkey; Central nervous system; NMR spectrometry; Metabolism; Carbon; In vivo; Vertebrata; Mammalia; Aminoacid; Animal; Primates; Tridimensional image; Brain (vertebrata); Test object
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/(SICI)1522-2594(200002)43:2<200::AID-MRM6>3.0.CO;2-H

A method for spatially three‐dimensional (3D) localized two‐dimensional (2D) 1H‐13C correlation spectroscopy, localized HSQC, is proposed. This method has the following special feature in the preparation period. The 180°(13C) and 180°(1H) pulses are separated in time, and the 180°(13C) pulse is applied at 1/(4 1JCH) before the 90°(1H) polarization transfer pulse. The preparation (echo) period 2τ can then be set substantially longer than 1/(2 1JCH), so that even in a whole‐body system, slice‐selective 90°(1H) pulses and gradient pulses can be applied in that period. The localization capabilities of this method were confirmed in a phantom experiment. The 3D localized 2D 1H‐13C correlation spectra from a monkey brain in vivo were obtained after [1‐13C]glucose injection, and amino acid metabolism was detected; that is, [4‐13C]glutamate appeared immediately after the injection, followed by the appearance of [2‐13C]glutamate, [3‐13C]glutamate, and [4‐13C]glutamine. Magn Reson Med 43:200–210, 2000. © 2000 Wiley‐Liss, Inc.