Nanosecond UV lasers stimulate transient Ca2+ elevations in human hNT astrocytes

• Published in: Journal of neural engineering Vol. 14; no. 3; p. 035001
• Main Authors: Raos, B J, Graham, E S, Unsworth, C P
• Format: Journal Article
• Language: English
• Published: IOP Publishing 01.06.2017
• Subjects: astrocyte; calcium; nanosecond laser; NT2; NTERA2; photo-stimulation
• ISSN: 1741-2560; 1741-2552
• DOI: 10.1088/1741-2552/aa5f27

Objective. Astrocytes respond to various stimuli resulting in intracellular Ca2+ signals that can propagate through organized functional networks. Recent literature calls for the development of techniques that can stimulate astrocytes in a fast and highly localized manner to emulate more closely the characteristics of astrocytic Ca2+ signals in vivo. Approach. In this article we demonstrate, for the first time, how nanosecond UV lasers are capable of reproducibly stimulating Ca2+ transients in human hNT astrocytes. Main results. We report that laser pulses with a beam energy of 4-29 µJ generate transient increases in cytosolic Ca2+. These Ca2+ transients then propagate to adjacent astrocytes as intercellular Ca2+ waves. Significance. We propose that nanosecond laser stimulation provides a valuable tool for enabling the study of Ca2+ dynamics in human astrocytes at both a single cell and network level. Compared to previously developed techniques nanosecond laser stimulation has the advantage of not requiring loading of photo-caged or -sensitising agents, is non-contact, enables stimulation with a high spatiotemporal resolution and is comparatively cost effective.