Exophiala

The composition of the genus Exophiala has undergone signi–cant revisions following recent application of molecular techniques [4-6]. For example, E. jeanselmei is a well-known heterogeneous, cryptic species, which contains three varieties (E. jeanselmei var. lecanii-corni, E. jeanselmei var. hetero...

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Bibliographic Details
Published inMolecular Detection of Human Fungal Pathogens pp. 274 - 281
Format Book Chapter
LanguageEnglish
Published United Kingdom CRC Press 2011
Taylor & Francis Group
Subjects
FAMILY, HOME AND PRACTICAL INTERESTS
Food & beverage technology
Molecular biology
Exophiala Jeanselmei
Real Time PCR
μL Template DNA
GE Healthcare Bio-Sciences
Obsolete Synonyms
Sterile MilliQ Water
SAMPLE PREPARATION
Hortaea Werneckii
Gomori’s Methenamine Silver Stain
FastStart Taq DNA Polymerase
Sabouraud Agar
PDA
Subcutaneous Infections
IDI
CGT
Exophiala Dermatitidis
Tris EDTA Buffer
Strain CBS
Conidiogenous Cells
Primer ITS1
Lysis Tube
Te Buffer
Dematiaceous Fungi
PDA Slant
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Summary:The composition of the genus Exophiala has undergone signi–cant revisions following recent application of molecular techniques [4-6]. For example, E. jeanselmei is a well-known heterogeneous, cryptic species, which contains three varieties (E. jeanselmei var. lecanii-corni, E. jeanselmei var. heteromorpha, and E. jeanselmei) on the basis of its morphological features. Sequence analysis of E. jeanselmei rRNA internal transcribed spacer (ITS) and other gene regions revealed that the former E. jeanselmei species can be separated into E. heteromorpha, E. lecanii-corni, E. oligosperma, and E. xenobiotica plus E. jeanselmei [7]. Furthermore, molecular examination of the Exophiala spinifera-E. jeanselmei clade led to the differentiation of E. spinifera, E. jeanselmei, E. attenuata, and E. (Phaeococcomyces) exophialae as well as an unidenti–ed Exophiala sp. (represented by strain CBS 725.88) [8,9].
ISBN:1439812403
9781439812402
DOI:10.1201/b11375-35