In vitro and in vivo inhibitory effect of ethanol and acetaldehyde on O6-methylguanine transferase
Human and rat O6-methylguanine transferase (O6MeGT) are inhibited in vitro by ethanol at concentrations of 10 to 50 mM and by acetaldehyde, the first metabolite of ethanol, at concentrations as low as 0.01 microM. Several other enzymes, including glyceraldehyde-3-phosphate dehydrogenase and yeast al...
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Published in | Carcinogenesis (New York) Vol. 9; no. 5; p. 761 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
01.05.1988
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Subjects | |
Online Access | Get more information |
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Summary: | Human and rat O6-methylguanine transferase (O6MeGT) are inhibited in vitro by ethanol at concentrations of 10 to 50 mM and by acetaldehyde, the first metabolite of ethanol, at concentrations as low as 0.01 microM. Several other enzymes, including glyceraldehyde-3-phosphate dehydrogenase and yeast alcohol dehydrogenase, which like O6MeGT have cysteines in their active sites, were not inhibited by acetaldehyde at the levels that inhibited O6MeGT. Disulfiram, an acetaldehyde dehydrogenase inhibitor, enhanced the inhibitory effect of ethanol in vivo. These results indicate that the inhibitory effect of ethanol on O6MeGT activity is mediated primarily via its metabolite, acetaldehyde. |
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ISSN: | 0143-3334 |
DOI: | 10.1093/carcin/9.5.761 |