Amorphous TiO2 nano-coating on stainless steel to improve its biological response

• Published in: Biomedical materials (Bristol) Vol. 19; no. 5
• Main Authors: Garcia-Perez, Victor I, Hotchkiss, Kelly M, Silva-Bermudez, Phaedra, Hernández, Miryam Martínez, Prado-Prone, Gina, Olivares-Navarrete, Rene, Rodil, Sandra E, Almaguer-Flores, Argelia
• Format: Journal Article
• Language: English
• Published: IOP Publishing 21.08.2024
• Subjects: antibacterial surfaces; inflammatory cytokines; nanocoating; orthopaedic implants; osteogenesis
• ISSN: 1748-6041; 1748-605X
• DOI: 10.1088/1748-605X/ad6dc4

This study delves into the potential of amorphous titanium oxide (aTiO 2 ) nano-coating to enhance various critical aspects of non-Ti-based metallic orthopedic implants. These implants, such as medical-grade stainless steel (SS), are widely used for orthopedic devices that demand high strength and durability. The aTiO 2 nano-coating, deposited via magnetron sputtering, is a unique attempt to improve the osteogenesis, the inflammatory response, and to reduce bacterial colonization on SS substrates. The study characterized the nanocoated surfaces (SS-a TiO 2 ) in topography, roughness, wettability, and chemical composition. Comparative samples included uncoated SS and sandblasted/acid-etched Ti substrates (Ti). The biological effects were assessed using human mesenchymal stem cells (MSCs) and primary murine macrophages. Bacterial tests were carried out with two aerobic pathogens ( S. aureus and S. epidermidis ) and an anaerobic bacterial consortium representing an oral dental biofilm. Results from this study provide strong evidence of the positive effects of the aTiO 2 nano-coating on SS surfaces. The coating enhanced MSC osteoblastic differentiation and exhibited a response similar to that observed on Ti surfaces. Macrophages cultured on aTiO 2 nano-coating and Ti surfaces showed comparable anti-inflammatory phenotypes. Most significantly, a reduction in bacterial colonization across tested species was observed compared to uncoated SS substrates, further supporting the potential of aTiO 2 nano-coating in biomedical applications. The findings underscore the potential of magnetron-sputtering deposition of aTiO 2 nano-coating on non-Ti metallic surfaces such as medical-grade SS as a viable strategy to enhance osteoinductive factors and decrease pathogenic bacterial adhesion. This could significantly improve the performance of metallic-based biomedical devices beyond titanium.