Study on conformational states of Escherichia coli tRNAPhe in solution by a modulation-free ESR-spectrometer

A modulationfree Electron Spin Resonance spectrometer was used for the registration of spectral absorption lines of a spin-labeled Escherichia coli phenylalanine tRNA in solution with low (less than 0.1%) line shape distortion. The analysis of line shape of two different spin-labels introduced into...

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Bibliographic Details
Published inNucleic acids research Vol. 10; no. 3; pp. 1113 - 1126
Main Authors Bondarev, G.N., Isaev-Ivanov, V.V., Isaeva-Ivanova, L.S., Kirillov, S.V., Kleiner, A.R., Lepekhin, A.F., Odinzov, V.B., Fomichev, V.N.
Format Journal Article
LanguageEnglish
Published England Oxford University Press 11.02.1982
Subjects
Edetic Acid
Electron Spin Resonance Spectroscopy
Escherichia coli
Escherichia coli - chemistry
Hydrogen-Ion Concentration
Magnesium
Nucleic Acid Conformation
RNA, Transfer, Amino Acyl
Solutions
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Summary:A modulationfree Electron Spin Resonance spectrometer was used for the registration of spectral absorption lines of a spin-labeled Escherichia coli phenylalanine tRNA in solution with low (less than 0.1%) line shape distortion. The analysis of line shape of two different spin-labels introduced into position 8 revealed that phenylalanine tRNA in solution exists as a mixture of two conformers, the equilibria between conformers being dependent on pH, concentration of magnesium and functional state of tRNA (deacylated, aminoacylated or peptidylated). There are no overall structural rearrangements upon aminoacylation or peptidylation of tRNA. The observed small changes of spectral line shape can be assigned to shifts in conformational equilibria.
Bibliography:istex:B0804035AF87E34A750DACC608362DC75221E56B
ark:/67375/HXZ-KC9SFC2V-9
ArticleID:10.3.1113
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/10.3.1113