Polymer microarrays for surface plasmon resonance based sensors

Surface plasmon resonance spectroscopy (SPR) is used to detect pH changes in acidic solutions. SPR is an optical, label-free method for highly sensitive detection of refractive index changes. A microarray of pH-sensitive polymers, using poly(2-vinylpyridine) (P2VP) and poly(4-vinylpyridine) (P4VP),...

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Bibliographic Details
Published inIEEE SENSORS 2014 Proceedings pp. 2086 - 2088
Main Authors Kick, Alfred, Mertig, Michael
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.11.2014
Subjects
Gold
Plasmons
Polymers
Refractive index
Sensitivity
Sensors
Surface morphology
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Summary:Surface plasmon resonance spectroscopy (SPR) is used to detect pH changes in acidic solutions. SPR is an optical, label-free method for highly sensitive detection of refractive index changes. A microarray of pH-sensitive polymers, using poly(2-vinylpyridine) (P2VP) and poly(4-vinylpyridine) (P4VP), as recognition elements, is investigated on a gold surface. Swelling and protonation of the polymers lead to refractive index changes at the sensor surface. For the first time, a polymer microarray in a microfluidic channel is used to detect pH values smaller than 5 by SPR in aqueous hydrochloric acid and buffered solutions of different ionic strength. Every polymer has a distinct pH range where it shows its highest pH sensitivity. pH measurements are performed in a range between pH 0.75 and 5. The ionic strength influences the swelling behavior and the pH sensitivity of the polymers. The applied polymers are pH-selective in the used buffers containing sodium, phosphate, citrate, potassium and chloride ions.
ISSN:1930-0395
2168-9229
DOI:10.1109/ICSENS.2014.6985447