Preparation, Characterization and Fluorescent Immunoassay Application of Rubpy-Doped Silica Nanoparticles

A novel type of Rubpy-doped fluorescent nano-particles has been prepared using a water-in-oil(W/O) microemulsion method. Characterizations by transmission electron microscopy, fluorescent spectra, photostability experiments and dyeleaking experiments show that the nanoparticles are spherical, monodi...

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Bibliographic Details
Published in2010 4th International Conference on Bioinformatics and Biomedical Engineering pp. 1 - 4
Main Authors Dongguang Yin, Binhu Liu, Li Zhang, Chunjuan Xie, Le Zhang
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.06.2010
Subjects
Biomedical measurements
Chemical analysis
Fluorescence
Immune system
Immunity testing
Nanoparticles
Probes
Shape control
Silicon compounds
Transmission electron microscopy
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Summary:A novel type of Rubpy-doped fluorescent nano-particles has been prepared using a water-in-oil(W/O) microemulsion method. Characterizations by transmission electron microscopy, fluorescent spectra, photostability experiments and dyeleaking experiments show that the nanoparticles are spherical, monodisperse, and uniform in sharp and size (70 ± 5nm). Due to a lot of fluorescent dye molecules encapsulated in the silica matrix that also severs to protect Rubpy dye from photodamaging oxidation, the nanoparticles are extremely bright, photostable and chemical stable. The prepared nanoparticles have been used as fluorescent probe successfully in fluorescent immunoassay.
ISBN:9781424447121
1424447127
ISSN:2151-7614
2151-7622
DOI:10.1109/ICBBE.2010.5516455