Comparison of Stability for All-trans Retinoic Acid Nanosuspensions and Lipid Nanoparticle Formulations

• Published in: 2007 IEEE/ICME International Conference on Complex Medical Engineering pp. 197 - 202
• Main Authors: Ma, Q.H., Tong, S., Duan, L., Xia, Q., Gu, N.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 01.05.2007
• Subjects: All-trans retinoic acid; Chemical analysis; Drugs; Lipidomics; Nanoemulsion; Nanoparticles; Nanostructured lipid carriers; Nanostructured materials; Nanosuspension; Petroleum; Solid lipid nanoparticles; Solids; Solvents; Stability; Stability analysis; Testing
• ISBN: 9781424410774; 1424410770
• DOI: 10.1109/ICCME.2007.4381721

The principal aim of this study was to compare the stability of different nanoparticle formulations with lipophilic and chemically unstable model drug nW-trans retinoic acid (ATRA). ATRA nanoparticles (ATRA-NP) and ATRA-loaded solid lipid nanoparticles (ATRA-SLN, palmitic acid as solid lipid matrix) were prepared by solvent injection method, whilst ATRA-loaded nanostructured lipid carriers (ATRA-NLC, glyceryl monostearate and palmitic acid (3:1 w/w) as solid lipid matrix, soybean oil as liquid lipid material), ATRA-SLN (glyceryl monostearate as solid lipid matrix), and ATRA-loaded nanoemulsions (ATRA-NE, soybean oil as liquid lipid material) were successfully prepared by melt high pressure homogenization method. The chemical stability of ATRA was determined by HPLC analysis. It was found that ATRA in all tested colloidal carriers was more stable than that in methanol solution. The type of colloidal carries systems, preparation method, lipid phase state, concentration of lipid and drug had a strong influence on the chemical stability of ATRA. ATRA was more stable in SLN than in NP, which were both produced by solvent injection method. However, ATRA-NP produced by solvent injection method showed higher stability than ATRA-SLN produced by melt high pressure homogenization method. Additionally, ATRA was more stable when it was incorporated in SLN than that entrapped in NLC or NE. The concentration of lipid or drug also influenced the stability of ATRA. We conclude that ATRA-NP or ATRA-loaded lipid nanoparticle formulations can satisfy the needs for improving both the solubility and stability limitations of ATRA.