Construction of a New Universal Plant Overexpression Vector for cDNA Transformation

The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those function-important cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new univer...

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Bibliographic Details
Published in2010 4th International Conference on Bioinformatics and Biomedical Engineering pp. 1 - 4
Main Authors Dang-Quan Zhang, Zhen-Jun Gu, Shun-Yang Deng, Shao-Gang Fan, Quan-Dong Zhu
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.06.2010
Subjects
Biochemistry
Bioinformatics
DNA
Forestry
Genomics
In vitro
RNA
Sequences
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Tail
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Summary:The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those function-important cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application. The new engineered transgenic vector was based on the pCAMBIA vector backbone, which was inserted by five motifs, including a promoter, an enhancer, a Linker DNA fragment, a endoplasmic reticulum retention signal (KDEL), and a NOS-T DNA. The target cDNA can be directly inserted into the new universal vector to construct the workable transgenic plasmid according to the interesting need of transgenic users. The result reported here will promote the plant transgenic application of cDNAs.
ISBN:9781424447121
1424447127
ISSN:2151-7614
2151-7622
DOI:10.1109/ICBBE.2010.5517341