Microfluidic chamber with active suction ports for localized chemical stimulation of brain slices

We report a novel microfluidic chamber with active suction ports to locally deliver soluble neuromodulators into a brain slice in a precisely controlled manner. A two-level soft-lithography process is developed to fabricate injection/suction port assemblies integrated with microchannels. Fluorescenc...

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Bibliographic Details
Published in2010 IEEE 23rd International Conference on Micro Electro Mechanical Systems (MEMS) pp. 304 - 307
Main Authors Tang, Y Tanye, Jichul Kim, Lopez-Valdes, Hector, Brennan, K C, Ju, Y Sungtaek
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.01.2010
Subjects
Aerospace engineering
Assembly
Brain
Chemicals
Drugs
Extracellular
Fluorescence
Microchannel
Microfluidics
Nervous system
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Summary:We report a novel microfluidic chamber with active suction ports to locally deliver soluble neuromodulators into a brain slice in a precisely controlled manner. A two-level soft-lithography process is developed to fabricate injection/suction port assemblies integrated with microchannels. Fluorescence imaging is used to characterize the flows around an injection port while switching the suction port on and off. To demonstrate biological applicability of the chamber, we use a focal delivery of potassium chloride solution to induce cortical spreading depression (CSD) in mice brain slices.
ISBN:1424457610
9781424457618
ISSN:1084-6999
DOI:10.1109/MEMSYS.2010.5442504