Automating the quantification of membrane proteins under confocal microscopy

In a prior contribution, we described a semi-automated system that allowed a user to quantify the relative abundance of fluorescently labeled membrane proteins in confocal microscopy images. Here, we describe a step change in assay automation, enabled by explicitly casting the problem in terms of ma...

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Bibliographic Details
Published in2012 9th IEEE International Symposium on Biomedical Imaging (ISBI) pp. 776 - 779
Main Authors Vallotton, P., Payne, M., Bednarz, T., Domanski, L., James, D., Hughes, W., Changming Sun
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.05.2012
Subjects
Algorithm design and analysis
Biomembranes
circular shortest paths
Dijkstra
Drugs
Fluorescence
high content analysis
high content screening
Membrane tracing
Microscopy
Proteins
shortest paths
Sun
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Summary:In a prior contribution, we described a semi-automated system that allowed a user to quantify the relative abundance of fluorescently labeled membrane proteins in confocal microscopy images. Here, we describe a step change in assay automation, enabled by explicitly casting the problem in terms of mathematical graphs. This permitted to include extensive and relevant image information in the tracing process; something not possible when using the marginally faster traditional algorithms. These improvements bring us closer to an industrial strength membrane tracing assay suitable in a drug discovery and development environment.
ISBN:145771857X
9781457718571
ISSN:1945-7928
1945-8452
DOI:10.1109/ISBI.2012.6235663