Random distribution of biomaterials as a handling method on microarray applied to PCR, biosensors and high through-put screening

• Published in: 1st Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology. Proceedings (Cat. No.00EX451) pp. 29 - 32
• Main Authors: Murakami, Y., Nagai, H., Kikuchi, T., Yamamura, A., Idegami, K., Yanase, M., Yong-Sung Choi, Morita, Y., Tamiya, E.
• Format: Conference Proceeding
• Language: English
• Published: IEEE 2000
• Subjects: Biochemistry; Biological materials; Biomembranes; Biosensors; DNA; Energy exchange; Fluorescence; Micromachining; Molecular biophysics; Self-assembly
• ISBN: 9780780366039; 0780366034
• DOI: 10.1109/MMB.2000.893734

Describes a random distribution method to bring biomaterials into an array of sites on a chip. A mixture of biomolecules or a mixed suspension of particles immobilized with biomaterials was simply poured on an array and the components were randomly distributed. A microchamber array for PCR was developed by semiconductor microfabrication techniques. Amplification of pGFP was confirmed using a technique based on energy transfer of fluorescent dyes. Addition of BSA in PCR mixture enhances PCR efficiency. Furthermore, employing a special membrane made it possible to pick up the PCR products, Random distribution method is also applied to the construction of biosensor arrays. The authors first employed a two-step immobilization in which materials are not immobilized directly but indirectly via small support materials. In this case random distribution is called random fluidic self-assembly. This arranges the microparticles reducing the complication in the second step on the chip.