A quantitative autoradiographic study of nucleolus-associated RNA and DNA synthesis during the eclipse phase in Rous sarcoma virus-infected chicken fibroblasts

Functional and morphologic differences between the sensitivity of nucleoli of Rous sarcoma virus-transformed cells and that of newly infected cells to the action of actinomycin D (AD) have been demonstrated by quantitative light and electron microscope autoradiography and utilized to investigate the...

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Bibliographic Details
Published inJNCI : Journal of the National Cancer Institute Vol. 54; no. 2; p. 349
Main Authors Suskind, R G, Michelson-Fiske, S, Haguenau, F, Rabotti, G F
Format Journal Article
LanguageEnglish
Published United States 01.02.1975
Subjects
Animals
Autoradiography
Avian Sarcoma Viruses
Binding Sites
Cell Nucleolus - drug effects
Cell Nucleolus - metabolism
Cell Transformation, Neoplastic
Cells, Cultured
Chick Embryo
Dactinomycin - metabolism
Dactinomycin - pharmacology
DNA, Neoplasm - biosynthesis
Fibroblasts - ultrastructure
RNA, Neoplasm - biosynthesis
Time Factors
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Summary:Functional and morphologic differences between the sensitivity of nucleoli of Rous sarcoma virus-transformed cells and that of newly infected cells to the action of actinomycin D (AD) have been demonstrated by quantitative light and electron microscope autoradiography and utilized to investigate the function of the nucleolus in the early stages of infection. After a pulse exposure to low doses of AD, increased RNA synthesis is induced within 80 minutes in the fibrillar portion of the nucleolus by infection. A concomitant increase in the retention of tritiated AD in the nucleolus and a quantitative redistribution of intranuclear and cytoplasmic DNA label are interpreted as evidence for a virus-induced amplification of the binding sites of AD in nucleolar chromatin.
ISSN:0027-8874
DOI:10.1093/jnci/54.2.349