HPLC determination of pka value of 10-hydroxy-2-decenoic acid

The utilization of HPLC methods is very useful for the determination of some physicochemical characteristics of compounds. In the current work, an analytical HPLC method is applied for the determination of pK a values and lipophilicity parameter of 10-HDA. The retention factor of the investigated co...

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Bibliographic Details
Published inJournal of liquid chromatography & related technologies Vol. 41; no. 2; pp. 87 - 92
Main Authors Angelov, T., Hristov, I.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 20.01.2018
Taylor & Francis Ltd
Subjects
10-Hydroxy-2-decenoic acid
Biological activity
High-performance liquid chromatography
HPLC determination
Lipophilicity
Liquid chromatography
pH effects
Process parameters
Retention
royal jelly
value
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Summary:The utilization of HPLC methods is very useful for the determination of some physicochemical characteristics of compounds. In the current work, an analytical HPLC method is applied for the determination of pK a values and lipophilicity parameter of 10-HDA. The retention factor of the investigated compound is determined at different pH ranges of the mobile phase. The derived mathematical description of the dependency of retention factor of pH is a sigmoid curve. The second derivative corresponds to the pK a of the investigated compound. The pK a and lipophilicity parameter values are the most important parameters which characterize the processes of penetration of biological active substances through the cellular membrane under different conditions.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2017.1408648