Increased expression of mRNA for regulator of G protein signaling domain–containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats

• Published in: American journal of hypertension Vol. 17; no. 10; pp. 981 - 985
• Main Authors: Ying, Zhekang, Jin, Liming, Dorrance, Anne M., Webb, R. Clinton
• Format: Journal Article
• Language: English
• Published: New York, NY Oxford University Press 01.10.2004; Elsevier Science
• Subjects: Arterial hypertension. Arterial hypotension; Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Clinical manifestations. Epidemiology. Investigative techniques. Etiology; Experimental diseases; G protein coupled receptor; Medical sciences; regulator of G protein signaling domain containing; Rho guanine nucleotide exchange factors; RhoA; stroke-prone spontaneously hypertensive rat; vascular smooth muscle; Hypertension; Nervous system diseases; Stroke; vascular smooth muscle; Rat; Rodentia; Cardiovascular disease; Smooth muscle; Rho guanine nucleotide exchange factors; RhoA; Cerebral disorder; Vascular disease; Vertebrata; stroke-prone spontaneously hypertensive rat; Mammalia; G protein coupled receptor; Animal; regulator of G protein signaling domain containing; Central nervous system disease; Aorta; Cerebrovascular disease; G protein
• ISSN: 0895-7061; 1879-1905
• DOI: 10.1016/j.amjhyper.2004.05.006

Background: Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). Methods: We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Results: Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. Conclusion: The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension.