Increased expression of mRNA for regulator of G protein signaling domain–containing Rho guanine nucleotide exchange factors in aorta from stroke-prone spontaneously hypertensive rats
Background: Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G...
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Published in | American journal of hypertension Vol. 17; no. 10; pp. 981 - 985 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Oxford University Press
01.10.2004
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Background: Rho guanine nucleotide exchange factors (RhoGEF) link activation of G protein-coupled receptors (GPCR) to RhoA/Rho-kinase signaling. This cellular signaling pathway regulates vascular tone and is implicated in hypertension. The RhoGEF, providing this coupling, contain the regulator of G protein signaling (RGS) domain. We hypothesized higher mRNA expression levels for these RhoGEF in aorta from stroke-prone spontaneously hypertensive rats (SHRSP). Methods: We measured mRNA expression by semiquantitative reverse transcriptase-polymerase chain reaction in the aorta of Wistar-Kyoto rats (WKY) and SHRSP. The protein expression level of p115RhoGEF was examined by Western Blot, too. Results: Our results showed that mRNA for RGS domain containing RhoGEF is higher in SHRSP. Conclusion: The results of this study indicate that RhoGEF may contribute to increased activation of RhoA/Rho-kinase pathway in hypertension. |
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Bibliography: | istex:ED14E628850128CA7F21B8F3D2C34767D97C206A ark:/67375/HXZ-N1F9JCXG-7 This study was supported by grants HL-18575 and HL-71138 from the National Institutes of Health (Bethesda, Maryland) and grant 0130364N from the American Heart Association (Chicago, Illinois). href:17_10_981.pdf |
ISSN: | 0895-7061 1879-1905 |
DOI: | 10.1016/j.amjhyper.2004.05.006 |