Similar rate of O6-ethylguanine elimination from DNA in normal human fibroblast and xeroderma pigmentosum cell strains not transformed by SV40

Using a highly specific rabbit antiserum (E3) directed against O6-ethyldeoxyguanosine in a competitive radioimmunoassay, we have measured the kinetics of removal of this alkylation product from the DNA of human fibroblasts treated with the N-nitroso carcinogen N-ethyl-N-nitrosourea. A very similar r...

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Bibliographic Details
Published inCarcinogenesis (New York) Vol. 4; no. 8; p. 1075
Main Authors Teo, I A, Lehmann, A R, Müller, R, Rajewsky, M F
Format Journal Article
LanguageEnglish
Published England 01.08.1983
Subjects
Cell Line
Cell Transformation, Neoplastic
DNA - metabolism
Fibroblasts - metabolism
Guanine - analogs & derivatives
Guanine - metabolism
Humans
Kinetics
Reference Values
Simian virus 40 - genetics
Skin - metabolism
Xeroderma Pigmentosum - metabolism
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Summary:Using a highly specific rabbit antiserum (E3) directed against O6-ethyldeoxyguanosine in a competitive radioimmunoassay, we have measured the kinetics of removal of this alkylation product from the DNA of human fibroblasts treated with the N-nitroso carcinogen N-ethyl-N-nitrosourea. A very similar rate of elimination from DNA was found in two normal human fibroblast strains (GM730 and 54BR) and in the nonvirus-transformed xeroderma pigmentosum fibroblast strains XP2BI and XP3BR. This is in contrast to the SV40-transformed xeroderma pigmentosum fibroblast strain XP12RO-SV40 which had previously been shown to be defective in its ability to remove O6-ethylguanine from DNA.
ISSN:0143-3334
DOI:10.1093/carcin/4.8.1075