Manipulation of DNA molecule utilizing the conformational transition in the higher order structure of DNA

We propose a new methodology on noncontact transportation of DNA molecules by dielectrophoretic force together with the micro DNA flow system. We utilize the conformational transition in the higher order structure of DNA for transportation. We have designed a simple micro electrode-flow system. Expe...

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Bibliographic Details
Published in1997 IEEE International Conference on Robotics and Automation Vol. 2; pp. 1454 - 1459 vol.2
Main Authors Morishima, K., Fukuda, T., Arai, F., Yoshikawa, K.
Format Conference Proceeding
LanguageEnglish
Japanese
Published IEEE 22.11.2002
Subjects
Bioinformatics
Coils
Conductivity
Dielectrophoresis
DNA
Electrodes
Electrokinetics
Genomics
Humans
Transportation
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ISBN9780780336124
0780336127
DOI10.1109/ROBOT.1997.614343

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Summary:We propose a new methodology on noncontact transportation of DNA molecules by dielectrophoretic force together with the micro DNA flow system. We utilize the conformational transition in the higher order structure of DNA for transportation. We have designed a simple micro electrode-flow system. Experimental demonstrations of DNA transportation in the globule state using dielectrophoretic force and direct observation of the DNA molecule in a nonuniform electric field were carried out with fluorescence microscopy. We discuss the experimental results on the motion of the DNA molecule. We show that transportation of DNA with the state of compacted globule is profitable in the future practical application for the separation of giant DNAs such as the human gene.
ISBN:9780780336124
0780336127
DOI:10.1109/ROBOT.1997.614343