Precursor of Rat Liver α2u-Globulin: Partial Amino Acid Sequence Determination of Its Signal Peptide

The biosynthesis of α2u-globulin, the major protein in rat urine, was studied in a cell-free system programmed with messenger RNA isolated from rat liver. Analysis of the primary translation product by SDS-polyacrylamide gel electrophoresis showed a molecular weight of approximately 22,000 daltons a...

Full description

Saved in:
Bibliographic Details
Published inJournal of biochemistry (Tokyo) Vol. 90; no. 6; pp. 1833 - 1836
Main Authors IKEHARA, Yukio, ODA, Kimimitsu, ROSENFELD, Melvin G., BAR-NUN, Shoshana, KREIBICH, Gert
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.10.1981
Online AccessGet full text

Cover

More Information
Summary:The biosynthesis of α2u-globulin, the major protein in rat urine, was studied in a cell-free system programmed with messenger RNA isolated from rat liver. Analysis of the primary translation product by SDS-polyacrylamide gel electrophoresis showed a molecular weight of approximately 22,000 daltons as compared to 20,000 daltons for the mature form. When translation was carried out in the presence of dog pancreas microsomes, α2u-globulin was cotranslationally processed and sequestered in the microsomal lumen. Automated sequencing to mature α2u and the labeled precursor disclosed the presence of 19 additional amino acids at the amino- terminal end of the primary translation product of the urinary α2u The partial amino acid sequence of this extension was determined.
Bibliography:istex:86CE1048E008340B28725948A0A0AB66F8609239
ark:/67375/HXZ-F20TKC6D-G
ArticleID:90.6.1833
1This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan, and by Grants GM 21971 and GM 20277 from the N.I.H. Melvin G. Rosenfeld was the recipient of United States Public Health Service Post-doctoral Fellowship GM 07177.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a133664