Detecting correlative light and electron microscopic labels by electron spectroscopic imaging

To bridge the gap in resolution and to localize molecules of interest in high-resolution light and electron microscopy a number of correlative approaches have been proposed. One possibility is the use of correlative markers, e.g. fluorescent Quantum Dots (Qdots), which in TEM are imaged and distingu...

Full description

Saved in:
Bibliographic Details
Published in2012 9th IEEE International Symposium on Biomedical Imaging (ISBI) p. 1320
Main Authors Pfannmoller, M., Roder, I. V., Benner, G., Stierhof, Y., Wacker, I., Schroder, R. R.
Format Conference Proceeding
LanguageEnglish
Published IEEE 01.05.2012
Subjects
Educational institutions
Electron microscopy
Energy loss
Fluorescence
Resins
Online AccessGet full text

Cover

More Information
Summary:To bridge the gap in resolution and to localize molecules of interest in high-resolution light and electron microscopy a number of correlative approaches have been proposed. One possibility is the use of correlative markers, e.g. fluorescent Quantum Dots (Qdots), which in TEM are imaged and distinguished by their contrast and shape. In 2007 Mhawi et al. demonstrated a more direct possibility, the visualization of electron low energy loss, which represents the TEM equivalent of fluorescence excitation. Such spectral information in the low energy-loss range was used to identify doxorubicin introduced into cells to label nuclei.
ISBN:145771857X
9781457718571
ISSN:1945-7928
1945-8452
DOI:10.1109/ISBI.2012.6235806