Surface enhanced Raman spectroscopy for characterization of structural characteristics of carbon chains in alpha1-acid glycoprotein and pseudoglycoproteins

Surface-enhanced Raman scattering (SERS) spectroscopy was used to study the structure of carbohydrate chains in glycosylated forms of alpha 1-acid glycoprotein (AGP) and in pseudoglycoproteins obtained by transferring the carbohydrate chains of AGP to a polyacrylamide carrier. It was found that AGP-...

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Bibliographic Details
Published inBioorganicheskaia khimiia Vol. 24; no. 6; p. 412
Main Authors Oleĭnikov, V A, Feofanov, A V, Shiian, S D, Tuzikov, A B, Kriukov, E Iu, Ianul', A I, Bovin, N B, Nabiev, I R
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.06.1998
Subjects
Acrylic Resins - chemistry
Adjuvants, Immunologic - chemistry
Carbohydrate Sequence
Carbohydrates - chemistry
Humans
Molecular Sequence Data
Orosomucoid - chemistry
Peptides - chemistry
Protein Conformation
Spectrum Analysis, Raman - methods
Structure-Activity Relationship
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Summary:Surface-enhanced Raman scattering (SERS) spectroscopy was used to study the structure of carbohydrate chains in glycosylated forms of alpha 1-acid glycoprotein (AGP) and in pseudoglycoproteins obtained by transferring the carbohydrate chains of AGP to a polyacrylamide carrier. It was found that AGP-D glycoform and pseudoglycoproteins containing three or more glycans per molecule, which possess high immunomodulating activity, have a specific spatial organization of carbohydrate chains. This organization is maintained by the interaction of neighboring glycans with each other and does not depend on the nature of the carrier (whether it is polypeptide or polyacrylamide).
ISSN:0132-3423