Overpassing an aberrant V(kappa) gene to sequence an anti-idiotypic abzyme with (beta)-lactamase-like activity that could have a linkage with autoimmune diseases

• Published in: The FASEB journal Vol. 15; no. 3; p. 815
• Main Authors: Debat, H, Avalle, B, Chose, O, Sarde, C O, Friboulet, A, Thomas, D
• Format: Journal Article
• Language: English
• Published: United States 01.03.2001
• Subjects: Amino Acid Sequence; Animals; Antibodies, Anti-Idiotypic - genetics; Antibodies, Anti-Idiotypic - metabolism; Antibodies, Catalytic - chemistry; Antibodies, Catalytic - immunology; Antibodies, Catalytic - metabolism; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - metabolism; Base Sequence; Cell Line; Cloning, Molecular; Electrophoresis, Agar Gel; Genes, Immunoglobulin; Immunoglobulin Heavy Chains - genetics; Immunoglobulin kappa-Chains - genetics; Immunoglobulin kappa-Chains - metabolism; Kinetics; Mice; Molecular Sequence Data; Polymerase Chain Reaction - methods; RNA - metabolism; Sequence Alignment
• ISSN: 0892-6638
• DOI: 10.1096/fj.00-0410com

A monoclonal antibody 9G4H9 that exhibits a beta-lactamase-like activity was previously obtained in accordance with the idiotypic network theory. This abzyme presents the most catalytic efficiency in amidase activity described in literature (kcat = 0.9 min-1). Some reports have demonstrated that functionality as complex as catalysis may be mimicked in this way. Comparison of the catalytic properties of both enzyme and abzyme previously allowed us to obtain better knowledge about 9G4H9 abzymatic machinery. In attempt to characterize this abzyme, the variable regions of kappa and heavy chain were cloned. We present a 'universal' method to clone the correct Vkappa gene to bypass aberrant Vkappa (abVkappa) produced by MOPC-21-derived hybridomas. Sequences obtained are compared in the GenBank database. The VH and Vkappa genes present some important sequence homology with autoantibodies suggesting a direct relationship between catalytic anti-idiotypic antibody and autoimmunity.