Plant Defense-related Transcription Factor WRKY6 Plays Both Supportive and Inhibitory Roles in Tobacco mosaic virus Infection

• Published in: Plant pathology bulletin Vol. 19; no. 1; pp. 031 - 040
• Main Authors: Chen, C-E, Yeh, H-H
• Format: Journal Article
• Language: Chinese
• Published: 台灣 中華民國植物病理學會 01.03.2010
• Subjects: Arabidopsis thaliana; Microarray; Tobacco mosaic virus; WRKY6; 菸草嵌紋病毒; 阿拉伯芥
• ISSN: 1021-9544

病毒侵染寄主時，是否可以成功進行複製並完成生活史乃是取決於和寄主產生的交互作用。為了瞭解病毒感染早期所調控的寄主反應，我們以Microarray系統的分析菸草嵌紋病毒(Tobacco mosaic virus, TMV)感染初期(4小時) 會對阿拉伯芥的轉錄體造成哪些影響？結果顯示約 2% 的寄主基因在 TMV 感染初期有被顯著的調控(變化大於2倍)而僅有8個基因的變化大於3倍，8個基因中和轉錄調控相關的基因佔了4個。進一步挑選其中一個目標基因 WRKY6 transcription factor 進行分析。於先前研究已知，WRKY6和許多植物生理功能的調節相關，包括植物的抗性反應、發育以及植物對磷、硼元素缺乏的敏感性皆會受 WRKY6影響。在TMV感染 WRKY6大量表現或抑制表現的原生質體中，TMV於24小時內的累積量未受到明顯影響；但TMV的累積量在WRKY6大量表現或抑制表現的植物中皆會受到抑制。顯示WRKY6並非是病毒感染初期的所需因子，但WRKY6在植物中同時扮演了促進和抑制病毒感染的角色
The initial stage of crosstalk between a virus and host may determine the successful replication and further infection by the virus. To understand the early stage of host gene regulation in response to viral infection, we used a microarray assay to analyze protoplasts of Arabidopsis thaliana 4 hr after their inoculation with Tobacco mosaic virus (TMV). Approximately only 2% of the host genes showed greater than 2-fold changed expression, and only 8 genes showed greater than 3-fold changed expression. Four of the 8 genes are involved in transcriptional regulatio