Molecular Cloning of Sigma Factor Gene of Phytoplasma Associated with Peanut Witches' Broom

• Published in: Plant pathology bulletin Vol. 17; no. 4; pp. 279 - 287
• Main Authors: 陳紹寬(Chen, Shau-kwaun), 朱佩文(Chu, Pei-wen), 何國傑(Ho, Kuo-chieh), 陳武揚(Chen, Wuu-yang), 林長平(Lin, Chan-pin)
• Format: Journal Article
• Language: Chinese
• Published: 台灣 中華民國植物病理學會 01.12.2008
• Subjects: Arachis hypogaea; Mollicutes; Mollicutes綱; PCR-based cloning; RNA polymerase; RNA聚合?; rpoD gene; rpoD基因; 聚合?連鎖反應選殖策略
• ISSN: 1021-9544

To elucidate the molecular biology of phytoplasmas, a gene encoding the primary sigma factor of the phytoplasma associated with peanut witches' broom (PnWB) was cloned and sequenced. One complete open reading frame (ORF) was determined on the insert of the selected recombinants. The ORF encodes a polypeptide contains -10 recognition and binding region, -35 recognition and binding region, core binding region and rpoD box. The gene organization and the nucleotide sequence in conserved region of the ORF are similar to other prokaryotic rpoD homologous sigma factor genes. According to the nucleic acid and amino acid sequence analyses, along with the results of Northern hybridization analyses, this gene was identified as a putative sigma factor gene. Based on the results of Southern hybridization analyses, it suggests that three rpoD homologous sigma factor genes may exist in PnWB phytoplasma.