Nucleotide Sequence Analysis of the Coat Protein Genes of Two Isolates of Sweet Potato Feathery Mottle Virus from Central Taiwan

• Published in: Plant pathology bulletin Vol. 16; no. 4; pp. 203 - 213
• Main Authors: 王麗媛(Wang, Li-yuan), 陳冠君(Chen, Kuan-chun), 陳宗祺(Chen, Tsung-chi), 葉錫東(Yeh, Shyi-dong)
• Format: Journal Article
• Language: Chinese
• Published: 台灣 中華民國植物病理學會 01.12.2007
• Subjects: Aphididae; Chenopodium quinoa; Nucleic acid cloning; Sequence analysis; Solanum tuberosum; Sweet potato feathery mottle virus; 序列分析; 核酸選殖; 甘藷羽狀斑駁病毒
• ISSN: 1021-9544

Two potyvirus-like isolates, CY1 and CY2, were collected from sweet potato displaying leaf symptoms of mosaic or vein mottling at Chia-Yi area, Taiwan, by single-lesion isolation on Chenopodium quinoa plants. Using the degenerate primers for potyviruses, a 1.2 kb and a 1.3 kb DNA fragments were amplified from CY1- and CY2-infected tissues of C. quinoa, respectively, by reverse-transcription polymerase chain reaction (RT-PCR). After cloning and sequencing, the two cDNA fragments were found to be of 1205 and 1351 bp, and corresponding to a part of the 3' end of nuclear inclusion (NIb) region and the 5' end of the coat protein (CP) region of potyviruses. For amplification of the region corresponding to the 3' end of CP region, the 3' untranslated region (3'-UTR), and the poly A tail, RT-PCR was conducted with the oligo(dT) primer and the specific primers designed from the known sequence. The assembled cDNA sequences of 1249 and 1383 bp, respectively, from CY1 and CY2 were elucidated to reflect the 3'-terminal re