Pathogenic role of anti–signal recognition protein and anti–3‐Hydroxy‐3‐methylglutaryl‐CoA reductase antibodies in necrotizing myopathies: Myofiber atrophy and impairment of muscle regeneration in necrotizing autoimmune myopathies

• Published in: Annals of neurology Vol. 81; no. 4; pp. 538 - 548
• Main Authors: Arouche‐Delaperche, Louiza, Allenbach, Yves, Amelin, Damien, Preusse, Corinna, Mouly, Vincent, Mauhin, Wladimir, Tchoupou, Gaelle Dzangue, Drouot, Laurent, Boyer, Olivier, Stenzel, Werner, Butler‐Browne, Gillian, Benveniste, Olivier
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2017; Wiley
• Subjects: Antibodies; Atrophy; Atrophy - pathology; Autoantibodies - immunology; Autoimmune Diseases - immunology; Autoimmune Diseases - pathology; Autoimmune Diseases - physiopathology; Biopsy; Cell culture; Cell Culture Techniques; Chains; Correlation; Cytokines; Damage; Fibers; Human health and pathology; Humans; Hydroxymethylglutaryl CoA Reductases - immunology; Hydroxymethylglutaryl-CoA reductase; Immunology; Impairment; In vitro methods and tests; Inflammation; Interleukin 13; Interleukin 4; Life Sciences; Molecular modelling; Muscle Fibers, Skeletal - immunology; Muscle Fibers, Skeletal - pathology; Muscle Fibers, Skeletal - physiology; Muscular Diseases - immunology; Muscular Diseases - pathology; Muscular Diseases - physiopathology; Myosin; Necrosis - pathology; Neonates; Oxygen; Pathology; Patients; Reactive oxygen species; Recognition; Regeneration; Regeneration - physiology; Signal Recognition Particle - immunology; Surface area; Tissue Banks; Transcription; Tumor necrosis factor
• ISSN: 0364-5134; 1531-8249
• DOI: 10.1002/ana.24902

Objective Immune‐mediated necrotizing myopathies (IMNM) may be associated with either anti–signal recognition protein (SRP) or anti–3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR) antibodies (Abs), and the titer of these Abs is correlated with disease activity. We investigated whether anti‐SRP and anti‐HMGCR Abs could be involved in muscle damage. Methods Muscle biopsies of patients were analyzed for atrophy and regeneration by measuring fiber size and by performing immunostaining of neonatal myosin heavy chain. To further understand the role of the Abs in the pathology, we performed muscle cell coculture with the Abs. Atrophy and regeneration were evaluated based on the myotube surface area as well as gene and cytokine profiles. Results In muscle biopsies of patients with anti‐SRP+ and anti‐HMGCR+ Abs, a large number of small fibers corresponding to both atrophic and regenerating fibers were observed. In vitro, anti‐SRP and anti‐HMGCR Abs induced muscle fiber atrophy and increased the transcription of MAFbx and TRIM63. In addition, the muscle fiber atrophy was associated with high levels of inflammatory cytokines: tumor necrosis factor, interleukin (IL)‐6, and reactive oxygen species. In the presence of anti‐SRP or anti‐HMGCR Abs, mechanisms involved in muscle regeneration were also impaired due to a defect of myoblast fusion. This defect was associated with a decreased production of IL‐4 and IL‐13. The addition of IL‐4 and/or IL‐13 totally rescued fusion capacity. Interpretation These data show that molecular mechanisms of atrophy and regeneration are affected and contribute to loss of muscle function occurring in IMNM. This emphasizes the potential interest of targeted therapies addressing these mechanisms. Ann Neurol 2017;81:538–548