De novo myofibrillogenesis in C2C12 cells: evidence for the independent assembly of M bands and Z disks

• Published in: American Journal of Physiology: Cell Physiology Vol. 290; no. 2; pp. C626 - C637
• Main Authors: Kontrogianni-Konstantopoulos, Aikaterini, Catino, Dawn H, Strong, John C, Bloch, Robert J
• Format: Journal Article
• Language: English
• Published: United States 01.02.2006
• Subjects: Actinin - metabolism; Actins - metabolism; Animals; Cell Line; Connectin; Guanine Nucleotide Exchange Factors - metabolism; Mice; Muscle Proteins - metabolism; Muscle, Skeletal - cytology; Muscle, Skeletal - growth & development; Muscle, Skeletal - metabolism; Myofibrils - physiology; Myofibrils - ultrastructure; Myosins - metabolism; Protein Kinases - metabolism
• ISSN: 0363-6143; 1522-1563
• DOI: 10.1152/ajpcell.00442.2005

Department of Physiology, School of Medicine, University of Maryland at Baltimore, Baltimore, Maryland Submitted 1 September 2005 ; accepted in final form 2 October 2005 We studied the distribution of the giant sarcomeric protein obscurin during de novo myofibrillogenesis in C 2 C 12 myotubes to learn when it is integrated into developing sarcomeres. Obscurin becomes organized first at the developing M band and later at the mature Z disk. Primordial M bands consisting of obscurin, myomesin, and M band epitopes of titin assemble before adult fast-twitch sarcomeric myosin is organized periodically and nearly concurrently with primitive Z disks, which are composed of -actinin and Z disk epitopes of titin. Z disks and M bands can assemble independently at spatially distant sites. As sarcomerogenesis proceeds, these structures interdigitate to produce a more mature organization. Fast-twitch muscle myosin accumulates in the myoplasm and assembles into A bands only after Z disks and M bands assume their typical interdigitated striations. The periodicities of M bands remain constant at 1.8 µm throughout sarcomerogenesis, whereas distances between Z disks increase from 1.1 µm in early sarcomeres to 1.8 µm in more mature structures. Our findings indicate for the first time that primitive M bands self-assemble independently of Z disks, that obscurin is a component of these primitive M bands in skeletal muscle cells, and that A bands assemble only after M bands and Z disks integrate into maturing sarcomeres. obscurin; titin; myosin; myomesin; -actinin; A band Address for reprint requests and other correspondence: A. Kontrogianni-Konstantopoulos, Dept. of Physiology, School of Medicine, Univ. of Maryland at Baltimore, 685 W. Baltimore St., Baltimore, MD 21201 (e-mail: akons001{at}umaryland.edu )