Helium-neon laser treatment transforms fibroblasts into myofibroblasts

• Published in: The American journal of pathology Vol. 137; no. 1; pp. 171 - 178
• Main Authors: Pourreau-Schneider, N, Ahmed, A, Soudry, M, Jacquemier, J, Kopp, F, Franquin, JC, Martin, PM
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01.07.1990; American Society for Investigative Pathology
• Subjects: 550600 > Medicine; Actins - analysis; ANIMAL CELLS; ANIMALS; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CELL DIFFERENTIATION; Cells, Cultured; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; ELECTRON MICROSCOPY; FIBROBLASTS; Fibroblasts - radiation effects; Fibroblasts - ultrastructure; GAS LASERS; HEALING; HELIUM-NEON LASERS; Humans; INJURIES; LASER RADIATION; LASERS; MAMMALS; MAN; Medical sciences; MICROSCOPY; Microscopy, Electron; Miscellaneous; PRIMATES; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RADIOLOGY AND NUCLEAR MEDICINE; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); RECOVERY; SOMATIC CELLS; VERTEBRATES 560400 > Other Environmental Pollutant Effects; Wound Healing; WOUNDS; Human; Immunohistochemistry; Pathology; Treatment; Myofibroblast; Laser; Instrumentation therapy; Exploration; Fibroblast
• ISSN: 0002-9440; 1525-2191

The differentiation of myofibroblastic cells from normal human gingival fibroblasts in vitro has been established by transmission electron microscopy and quantitated by immunohistochemistry, using antigelsolin monoclonal antibodies. Untreated control cultures were compared to cultures exposed to Helium-Neon (He-Ne) laser irradiation. A direct and massive transformation of the cultured fibroblasts into myofibroblasts was observed as early as 24 hours after laser treatment, whereas control cultures were comprised of only resting fibroblasts and active fibroblasts. This in vitro induction of myofibroblasts may be analogous to that which occurs in vivo. Therefore we undertook a similar study using biopsies from gingival tissues after wisdom tooth extraction. Myofibroblasts were present in the connective tissue of laser-treated gums 48 hours after irradiation, but not in untreated contralateral control tissues. These data provide evidence that the primary biologic effect of the Helium-Neon laser on connective tissue is the rapid generation of myofibroblasts from fibroblasts. The induction of a phenotype with contractile properties may have clinical significance in the acceleration of the wound-healing process.