The Major Yolk Protein of Sea Urchins Is Endocytosed by a Dynamin-Dependent Mechanism
Sea urchin oocytes grow to 10 times their original size during oogenesis by both synthesizing and importing a specific repertoire of proteins to drive fertilization and early embryogenesis. During the vitellogenic growth period, the major yolk protein (MYP), a transferrin-like protein, is synthesize...
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Published in | Biology of reproduction Vol. 71; no. 3; pp. 705 - 713 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.09.2004
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Subjects | |
Online Access | Get full text |
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Summary: | Sea urchin oocytes grow to 10 times their original size during oogenesis by both synthesizing and importing a specific repertoire
of proteins to drive fertilization and early embryogenesis. During the vitellogenic growth period, the major yolk protein
(MYP), a transferrin-like protein, is synthesized in the gut, transported into the ovary, and actively endocytosed by the
oocytes. Here, we begin to dissect this mechanism by first testing the hypothesis that MYP endocytosis is dynamin-dependent.
We have identified a sea urchin dynamin cDNA that is highly similar in amino acid sequence, structure, and size to mammalian
dynamin I: it contains an N-terminal GTPase domain, a pleckstrin-homology domain, and a C-terminal proline-rich domain. Sea
urchin dynamin is enriched at the cortex of oocytes and colocalizes to MYP endocytic vesicles at the oocyte periphery. To
test for a functional relationship between MYP endocytosis and dynamin, we used a dominant-negative human dynamin I mutant
protein containing an alteration within the GTPase domain (hDyn K44A ) to specifically compete for dynamin function. Using a fluorescent MYP construct to follow its endocytosis solely, as well
as a general endocytosis marker, we demonstrate that the disruption of dynamin function significantly reduces MYP uptake but
does not affect fluid-phase endocytosis. Using this specific biochemical approach, we are able to separate distinct pathways
of endocytosis during oogenesis and learn that dynamin-mediated endocytosis is responsible for MYP endocytosis but not fluid-phase
uptake. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.104.027730 |