Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes

The ultrastructural localization of the CD68 antigen, a 110-kd intracellular glycoprotein associated with myeloid cells and with monocytes/macrophages, was investigated in human neutrophil granulocytes by postembedding immunogold staining, using monoclonal antibody KP1. The antigen was found in the...

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Bibliographic Details
Published inThe American journal of pathology Vol. 139; no. 5; pp. 1053 - 1059
Main Authors Saito, N, Pulford, KA, Breton-Gorius, J, Masse, JM, Mason, DY, Cramer, EM
Format Journal Article
LanguageEnglish
Published Bethesda, MD ASIP 01.11.1991
American Society for Investigative Pathology
Subjects
Antibodies, Monoclonal
Antigens, CD - blood
Antigens, Differentiation, Myelomonocytic - blood
Biological and medical sciences
Cytoplasmic Granules - chemistry
Cytoplasmic Granules - ultrastructure
Epitopes - immunology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gold
Humans
Immunobiology
Immunohistochemistry - methods
Macrophages - chemistry
Macrophages - immunology
Microscopy, Electron
Monocytes - chemistry
Monocytes - immunology
Muramidase - analysis
Neutrophils - chemistry
Neutrophils - immunology
Peroxidase - analysis
Antigen
Human
Immunohistochemistry
Pathology
Monocyte
Pathophysiology
Exploration
Neutrophil
Localization
Blood
Macrophage
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Summary:The ultrastructural localization of the CD68 antigen, a 110-kd intracellular glycoprotein associated with myeloid cells and with monocytes/macrophages, was investigated in human neutrophil granulocytes by postembedding immunogold staining, using monoclonal antibody KP1. The antigen was found in the primary granules of neutrophils, although not all primary granules were labeled. It was absent from the plasma membrane. In monocytes, it was also detected within cytoplasmic granules, colocalized with lysozyme and myeloperoxidase. This observation confirms and completes results obtained by immunofluorescence and other light-microscopic methods. Moreover this study shows that the CD68 epitope recognized by antibody KP1 is able to resist fixation and embedment and therefore emphasizes the value of using KP1 as a marker for this macrophage-associated molecule.
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ISSN:0002-9440
1525-2191