Tumor cell growth fraction in Hodgkin's disease

• Published in: The American journal of pathology Vol. 128; no. 3; pp. 390 - 393
• Main Authors: Gerdes, J, Van Baarlen, J, Pileri, S, Schwarting, R, Van Unnik, JA, Stein, H
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01.09.1987; American Society for Investigative Pathology
• Subjects: Antibodies, Monoclonal; Antigens - immunology; Biological and medical sciences; Cell Division; Cell Nucleus - immunology; Hematologic and hematopoietic diseases; Histocytochemistry; Hodgkin Disease - pathology; Humans; Immunoenzyme Techniques; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Human; Immunohistochemistry; Double labelling; Hodgkin disease; Hemopathy; Monoclonal antibody; Kinetics; Sternberg cell; Tumor cell
• ISSN: 0002-9440; 1525-2191

The growth fraction of tumor cells was studied in 45 cases of Hodgkin's disease by means of a recently developed double immunostaining technique using monoclonal antibody Ki-1, which reacts selectively with Hodgkin and Reed-Sternberg cells in tissues affected by Hodgkin's disease, and antibody Ki-67, which recognizes a cell proliferation-associated nuclear antigen. The medians of the growth fractions of the tumor cells in all histologic subtypes of Hodgkin's disease varied between 78% and 83%. In none of the cases investigated did we find a growth fraction below 50%. Furthermore, mononucleated Hodgkin cells as well as multi-nucleated Reed-Sternberg cells showed a similar Ki-67 labeling index, indicating that both tumor cell types belong to the proliferating pool of this malignancy.