Rapid isolation of chromosomal breakpoints from patients with t(4;11) acute lymphoblastic leukemia : Implications for basic and clinical research

• Published in: Cancer research (Chicago, Ill.) Vol. 59; no. 14; pp. 3357 - 3362
• Main Authors: REICHEL, M, GILLERT, E, BREITENLOHNER, I, REPP, R, GREIL, J, BECK, J. D, FEY, G. H, MARSCHALEK, R
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.07.1999
• Subjects: Alu Elements; Binding Sites; Biological and medical sciences; Child; Child, Preschool; Chromosomes, Human, Pair 11 - genetics; Chromosomes, Human, Pair 11 - ultrastructure; Chromosomes, Human, Pair 4 - genetics; Chromosomes, Human, Pair 4 - ultrastructure; DNA Mutational Analysis; DNA Primers; DNA Repair; DNA Topoisomerases, Type II - metabolism; DNA, Neoplasm - genetics; DNA-Binding Proteins - genetics; Female; Hematologic and hematopoietic diseases; Histone-Lysine N-Methyltransferase; Humans; Infant; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Myeloid-Lymphoid Leukemia Protein; Nuclear Proteins - genetics; Polymerase Chain Reaction - methods; Precursor Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology; Prognosis; Proto-Oncogenes; Transcription Factors; Transcriptional Elongation Factors; Translocation, Genetic; Chromosomal aberration; Genetic mapping; Human; Breakpoint; Acute; Malignant hemopathy; Polymerase chain reaction; Chromosome break; Lymphoproliferative syndrome; Abnormal chromosome; Genetics; Acute lymphocytic leukemia; Tumor cell; Chromosome translocation
• ISSN: 0008-5472; 1538-7445

Chromosomal translocations t(4;11)(q21;q23) are associated with a group of acute lymphoblastic leukemias with very poor prognosis. From the complete sequences of the breakpoint cluster regions of the human MLL and AF-4 translocation partner genes, a novel set of 66 oligonucleotides that facilitates the rapid identification of translocation breakpoints by PCR analysis of genomic DNA was designed. For each breakpoint, a pair of optimally snited primers can be assigned, which improves the monitoring of the disease during treatment. Comparison of the breakpoints with the corresponding parental sequences also contributes to our better understanding of the illegitimate recombination events leading to these translocations.