Molecular characterization of circulating EBV DNA in the plasma of nasopharyngeal carcinoma and lymphoma patients

Despite the increasing clinical applications of circulating EBV DNA analysis as a tumor marker, the molecular nature of these EBV DNA molecules remains unclear. We subjected plasma/serum samples of nasopharyngeal carcinoma and lymphoma patients to DNase digestion and ultracentrifugation and showed t...

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Bibliographic Details
Published inCancer research (Chicago, Ill.) Vol. 63; no. 9; pp. 2028 - 2032
Main Authors ALLEN CHAN, K. C, JUN ZHANG, CHAN, Anthony T. C, LEI, Kenny I. K, LEUNG, Sing-Fai, CHAN, Lisa Y. S, CHOW, Katherine C. K, DENNIS LO, Y. M
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 01.05.2003
Subjects
Biological and medical sciences
Deoxyribonuclease I - metabolism
DNA, Viral - blood
Epstein-Barr virus
Epstein-Barr Virus Infections - complications
Epstein-Barr Virus Infections - genetics
Epstein-Barr Virus Infections - virology
Hematologic and hematopoietic diseases
Herpesvirus 4, Human - genetics
Hodgkin Disease - blood
Hodgkin Disease - virology
Humans
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymphoma, T-Cell - blood
Lymphoma, T-Cell - virology
Medical sciences
Nasopharyngeal Neoplasms - blood
Nasopharyngeal Neoplasms - virology
Otorhinolaryngology (head neck, general aspects and miscellaneous)
Otorhinolaryngology. Stomatology
Polymerase Chain Reaction
Tumors
Ultracentrifugation
Gammaherpesvirinae
Human
Biological fluid
Carcinoma
Herpesviridae
Biological marker
Malignant hemopathy
Nasopharynx
Epstein Barr virus
Malignant tumor
Blood plasma
Virus
Oncogenic virus
Lymphoproliferative syndrome
DNA
ENT disease
Malignant lymphoma
Pharynx disease
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Summary:Despite the increasing clinical applications of circulating EBV DNA analysis as a tumor marker, the molecular nature of these EBV DNA molecules remains unclear. We subjected plasma/serum samples of nasopharyngeal carcinoma and lymphoma patients to DNase digestion and ultracentrifugation and showed that circulating EBV DNA molecules are "naked" DNA fragments instead of being contained inside virions. We further showed that these EBV DNA fragments were relatively short, and 87% of them were shorter than 181 bp. These results provide fundamental information that may improve our understanding of the release of tumor-derived nucleic acids into the blood of cancer patients.
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ISSN:0008-5472
1538-7445