Identification and Characterization of an Ovary-Selective Isoform of Epoxide Hydrolase
A novel ovary-selective gene was identified by suppression subtractive hybridization (SSH) that is expressed only during the mouse periovulatory phase of a stimulated estrous cycle. Analysis of the protein encoded by the full-length cDNA revealed that the majority of it, with the exception of the fi...
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Published in | Biology of reproduction Vol. 72; no. 4; pp. 968 - 975 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Madison, WI
Society for the Study of Reproduction
01.04.2005
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Subjects | |
Online Access | Get full text |
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Summary: | A novel ovary-selective gene was identified by suppression subtractive hybridization (SSH) that is expressed only during the
mouse periovulatory phase of a stimulated estrous cycle. Analysis of the protein encoded by the full-length cDNA revealed
that the majority of it, with the exception of the first 44 amino acids, matched soluble epoxide hydrolase ( Ephx2, referred to as Ephx2A ). By comparing the cDNA sequence of this newly identified variant of soluble epoxide hydrolase (referred to as Ephx2B ) with the mouse genome database, an exon was identified that corresponds to its unique 5â² cDNA sequence. Through the use
of an Ephx2A -specific probe, Northern blot analysis revealed that this mRNA was also expressed in the ovary, with the highest level of
expression occurring during the luteal phase of a stimulated estrous cycle. In situ hybridization revealed that Ephx2B mRNA expression was restricted to granulosa cells of preovulatory follicles. Ephx2A mRNA expression, however, was detectable in follicles at different stages of development, as well as in the corpus luteum.
Total ovarian epoxide hydrolase activity increased following the induction of follicular development, and remained elevated
through the periovulatory and postovulatory stages of a stimulated estrous cycle. The change in enzyme activity paralleled
the combined mRNA expression profiles for both Ephx2A and Ephx2B, thus supporting a role for epoxide metabolism in ovarian function.
Abstract
Changes in enzyme activity parallel the combined mRNA expression profiles for both Ephx2A and Ephx2B, thus supporting a role for epoxide metabolism in ovarian function |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.104.035899 |